Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley

2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of purple-grained Tibetan hulless barley variety Nerumuzha and white-grained Kunlun 10. The HvnHID gene includes 981 bp open reading frame encodes a protein 327 amino acids. It has typical Abhydrolase_3 domain (78-306) belongs to carboxylesterase (CXE) family (α/β hydrolase) superfamily. There are eight nucleotide differences coding sequence two acid (one domain) between closest relationship with Hordeum vulgare, most distant Panicum hallii. At early-mid stage seed colour development, expression levels purple black were significantly higher than white blue ones (P < 0.01). During development barley, key genes (HvnF3'H, HvnDRF, HvnANT1, HvnGT) anthocyanidin biosynthetic pathway increased significantly, while decreased Thus, it is likely that negatively regulates This result provides basis for further study biological functions pathway.